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PCB 4233C Immunology

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Chapter 6 
You perform an Ouchterlony assay in which the central well contains goat antiserum against the F(ab')2 fragment of pooled mouse IgG and the surrounding wells (A-F) contain six different test antigens. The resulting precipitin pattern is show below. Based on this pattern, indicate which well contains each of the following test antigens:

Print this page and fill in  your answers--be prepared to discuss items in class. 

 

Well

Description 

a. 

Fab fragment from IgG myeloma protein (g2k2) 

b. 

Kappa (k) light chains 

c. 

Gamma (g) havy chains 

d. 

Lambda (l) light chains 

e. 

Fc fragment from IgG myeloma protein (g2k2) 

f. 

A mixture of g heavy chains and k light chains 

 

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