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PCB 4233C Immunology

Matching/Definitions/Terms

Chapter 2
Match each term related to recombinant DNA technology (a-i), select the most appropriate description (1-10) listed below. Each description may be used once, more than once, or not at all. You can utilize your book or link to Resources for help.   If there are descriptions which you do not use, find the term that you believe the description is associated with.

Print this page and fill in  your answers--be prepared to discuss items in class.

Terms

Description

Answer

a. Yeast artificial chromosome

1.  Cleaves mRNA at specific sites.

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b. Restriction endonuclease

2.  Cleaves double-stranded DNA at specific sites.

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c. cDNA

3.  Circular genetic element that can replicate in E.coli cells.

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d.  COS sites

4.  Used to clone DNA in mammalian cells.

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e. Retrovirus

5.  Formed from action of reverse transcriptase.

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f. Plasmid 

6.  Collection of DNA sequences within plasmid vectors representing all of the mRNA sequences derived from a cell.

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g. cDNA library

7.  Produced by action of certain DNA-cleaving enzymes.

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h. Sticky ends

8.  Used to clone very large DNA sequences.

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i. Genomic library

9.  Used to introduce larger-than-normal DNA fragments in l-phase vectors.

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10. Collection of l clones that includes all the DNA sequences of a given species.

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