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Section 1: General Properties
Learning Arenas
- Defining a virus
- Morphology and Structure
- Reproduction
- Effects of Physical, Chemical & Biological Agents
- Pathogenesis
Section 2: Taxonomy
Section 1: Safety Issues
Section 2: Laboratory Diagnosis
Learning Arenas
- Sampling and Processing
- Methods
- "Emerging" Viruses
Following the Modules outlined below, read the text chapters as indicated in one topic area at a time.
Review the Mycology slide atlas; Mycology colony atlas; Mycology structures and GermWare CD-Rom tutorial on the computers provided in the laboratory. If additional time is needed, the presentations can be checked out from the program executive secretary and viewed in the College of Health Professions or in the Computer Laboratory in Griffin Hall.
View microscopic slide preparations as indicated before proceeding onto the next area.
Read Module 2 and proceed with laboratory exercises. Schedule your laboratory exercises with the course instructor. This must be done at least one class session prior to the session you would like to perform the laboratory.
Upon completion of all laboratory exercises, set a time with the instructor to begin your exam practicum and written final. This must be done at least one class session prior to the session you would like to take the exams.
GermWare Mycology Tutorial and Reference CD-ROM
The GermWare Tutorial CD-ROM may be used in the laboratory during class or
may be checked out from the department executive secretary. The tutorial must
be viewed in the College of Health Professions or in the Computer Laboratory
(208 Griffin Hall). If the program software has not been installed on the
computer please ask the attendant for assistance.
Installing GermWare Mycology
To install GermWare, complete the following:
1. Start Microsoft Windows
2. Place your CD disc in the CD drive
3. Open the CD-ROM disc (or select Run from the Start menu, and click Browse)
4. Open the Setup Folder
5. Run Setup.exe
6. Select the default directories, and do a typical installation
WARNING: Do NOT do a custom installation!
7. Follow the instructions on the screen
Note: GermWare Mycology was created using Asymetrix Toolbook Instructor 11 Version 6. 1. See the License.wri file and the On-line Help System for licensing and copyright information. GermWare Mycology was developed using Asymetrix Assistant and Instructor 6.1 programs. During installation, the Toolbook Runtime files and the GermWare Mycology executable file will be installed to your hard disk. You must run GerrnWare with the GermWare CD in your computer's CD drive (the first CD drive, if you have multiple CD drives).
Using the GermWare Random Access Reference and Tutorial Programs
GermWare Mycology has been designed as an easy to use learning system. Various
means are available for accessing photographs and textual information for
61 species of medically significant molds and yeasts.
Note: First time users should use the Tutorial Program
Launching GermWare
Locate the GermWare Mycology Version 1.1 Group on your computer. If you are using Windows 95, use the START button and select Programs. GermWare Mycology will be a program option. Launch the application using the appropriate icon (you can launch either the reference or tutorial program).
The GermWare tutorial is a step-by-step introduction to Mycology, by module. Within each module, you will complete the tutorial in a linear fashion. The GermWare Reference System is a random access program where you may select any information within a given module.
System Navigation
In the GermWare Mycology Random Access Reference Program, multiple methods are provided for accessing its content. You may use either the buttons described below, or the right mouse menu to navigate within this program:
Global System Map
The Global System Map button takes you to a page that provides an overview listing of the 61 genera and species of fungi included in GermWare Mycology by taxonomic category. Click any fungus in the Global System Map to access information about the fungus.Information Page
The Information Page button will return you to the Information page for the current module. From the Information page you may select the System Map, Flow Diagram, Self-Assessment, Learning Objectives and Global Map.
The System Map
The System Map button will return you to the System Map on which are listed the various genera and species of fungi included in that current module.
The Flow Diagram
The Flow Diagram button will return you to the flow diagram for each of the modules, in which is included a decision tree on the practical steps necessary to identify each of the fungi included. More information can be obtained by clicking on any word or organism name that
is highlighted in red.
Additional GermWare Features
On-Line Glossary
The Glossary button is used to open the on-line glossary containing approximately 100 of the more commonly used terms. Once the glossary is open, select a letter of the alphabet to scroll the list of definitions to terms beginning with that letter. Click a term in the list to view its definition. Use the Close button to close the glossary when you are finished.The Image Comparison Feature
Included are comparison photomicrographs to help the user differentiate between certain fungal species and their look-alikes.Self-Assessment ProgramA Self-Assessment Program accompanies each module, composed of several one best response type questions. As each answer is selected, a dialog box provides immediate feedback on whether any given answer is correct, including a short explanation relative to the response. The user may return to the first question and to the Main Menu at any time during the examination.
Outline
I. Introduction to Mycology
II. Microscopic Morphology
III. Reproduction
IV. Colonial Morphology
Outline
I. Obtaining and Processing Specimens
II. Direct Examination of Specimens
A. Saline Wet MountB. Lactophenol Cotton Blue PrepartionsC. KOHD. Gram StainE. Acid-Fast StainF. Calcofluor White StainG. India Ink Preparations
III. Culture Procedures
A. Media
IV. Culture Examinations
A. Tease MountB. Cellophane TapeC. Slide Culture
V. Misc. Procedures
A. Skin TestsB. SerologyC. DNA TestingD. Antifungal Therapies
A. AcremoniumB. AlternariaC. A. clavatusD. A. flavusE. A. nigerF. A. terreusG. BipolarisH. ChaetomiumI. ChyrsosporiumJ. CurvulariaK. DrechsleraL. FusariumM. MucorN. PaecilomycesO. PenicilliumP. Pensillium chryseogenumQ. RhizopusR. ScedosporiumS. ScopulariopsisT. SepedoniumU. SyncephalastrumV. Ulocadium
III. Aseptate Opportunists
IV. Septate Opportunists
A. DematiacousB. Hyaline
V. Diseases
Outline
I. Superficial Organisms
II. Dermatophytes
A. MicrosporumB. EpidermophytonC. Trichophyton
III. Prepared Slides
A. M. audouiniiB. M. canisC. E. floccosumD. T. ajelloiE. T. mentagrophytesF. T. rubrumG. T. schoenleiniH. T. terrestre
A. Direct Examination
1. Culture2. Germ Tube TestB. Biochemical Identification
C. Microscopic Morphology
1. Prepared Slides
A. Candida albicans
B. Cryptococcus neoformans
C. Geotrichum candidium2. Corn Meal Agar
3. Niger Seed Agar
II. Diseases
A. DematiaceousB. Hyaline
II. Fungus-like Bacteria
III. Prepared Slides
A. NocardiaB. Philalophora verrucosaC. Sporothrix schenckiiD. Wangiella dermatitidis
A. Special Handling ProceduresB. Geographical Distribution
II. Blastomycoses
III. Coccidioidomycoses
IV. Histoplasmosis
V. Paracoccididioidomycoses
VI. Other
A. CryptococcosisB. Pneumocystis
Laboratories (100 Points): Laboratory exercises
are for your experience and learning. Schedule your laboratory exercises with
the course instructor. Scheduling must be done at least one class session
prior to the session you would like to perform the laboratory. The instructor
will demonstrate the correct procedures for the safe handling of mycology
specimens. Secure a laboratory procedure from the course instructor and lab
report form for these procedures. All laboratories must be completed and signed
off by the instructor prior to taking the final and practicum. Each specimen
processed and correctly identified is worth 50 points.
Obtain fungal specimens from instructor-minimum of 2 specimens randomly selected
Perform and record the following for each specimen:
Return to Mycology Progression
Final Practicum and Exam 300 points: The laboratory practicum consists of identification of fungal specimens by colonial morphology and microscopic identification. You will be able to use your notes during the microscopic examination. The written examination will consist of questions from the text and quizzes from the GermWare Mycology Tutorial. Upon completion of all laboratory exercises, set a time with the instructor to begin your exam practicum and written final. This must be done at least one class session prior to the session you would like to take the exams. The written exam may be scheduled separately from the practicums. All must be completed before progressing to the next unit.
Return to Parasitology Progression
Outline:
I. Introduction to Parasitology
II. Classification of Parasites
III. General Terminology
Outline:
I. Nematodes, Chapter 2
Prepared Slides Set A
II. Cestodes, Chapter 3
Prepared Slides Set B
III. Trematodes, Chapter 4
Prepared Slides C
Outline:
I. Pseudopodia
Prepared Slides Set D
II. Flagellates
Prepared Slides Set E
III. Ciliates
Prepared Slides Set F
IV. Sporozoa and Microsporidia
Prepared Slides Set G
Outline:
I. Insects and Arachnida
Prepared Slides Set H
Outline:
I. Fecal Examination
II. Macroscopic Examination
A. Microscopic Examination
B. Concentration Techniques
C. Trichrome Staining
D. Other Diagnostic Procedures
III. Blood Examination
A. Thick Smears
B. Thin Smears
Laboratories: Laboratory exercises are for your experience and learning. You may repeat these exercises as needed to gain competency. Schedule your laboratory exercises with the course instructor. Scheduling must be done at least one class session prior to the session you would like to perform the laboratory. Secure a laboratory procedure from the course instructor and lab form for these procedures. All laboratories must be completed and signed off by the instructor prior to taking the final and practicum.
1. Fecal ova and parasite examination-no fewer than 2 specimens
a. Macroscopic
b. Direct wet mount
c. Concentration
d. Trichrome staining
e. Microscopic examination and identification
2. Blood parasitic examinationa. Stain prepared slides with Wright's stain
b. Microscopic examination and identification
Final Practicum and Exam 400 points: The laboratory practicum consists of the processing of fecal specimens, examination and identification of unknown direct preparations, examination and identification of unknown prepared slides and a written exam. Upon completion of all laboratory exercises, set a time with the instructor to begin your exam practicum and written final. This must be done at least one class session prior to the session you would like to take the exams. The written exam may be scheduled separately from the practicums. All must be completed before progressing to the next unit.